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rabbit anti ptrkb  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti ptrkb
    Rabbit Anti Ptrkb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ptrkb/pm30211565__nl8b02317_si_001-280-6-9
    Average 86 stars, based on 1 article reviews
    rabbit anti ptrkb - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Estrogen administration attenuates post-stroke depression by enhancing CREB/BDNF/TrkB signaling in the rat hippocampus.
    Article Snippet: Protein (50 μg per lane) was loaded and separated via 12% SDS‐PAGE and transferred onto PVDF membranes (EMD Millipore). .. After blocking for 90 min at room temperature with 5% non‐fat dried milk in TBS, the membranes were incubated overnight at 4°C with anti‐β‐actin (1:1,000; cat. no. ab8226; Abcam), polyclonal anti‐CREB (1:5,000; cat. no. 9197; Cell Signaling Technology, Inc.), anti‐phospho‐CREB (1:5,000; cat. no. 9198; Cell Signaling Technology, Inc.) at Ser133, anti‐BDNF (1:1,000; cat. no. ab6201; Abcam), anti‐pTrkB (1:3,000; cat. no. 4168; Cell Signaling Technology, Inc.) or TrkB (1:3,000; cat. no. 4603; Cell Signaling Technology, Inc.) and shaken on a rotator at 4 ̊C for overnight. .. The membranes were washed three times with TBS containing 0.1% Tween‐20 (TBST) for 7 min each, and subsequently incubated in HRP‐labeled goat anti‐rabbit secondary antibody (1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) with TBST for 1 h at room temperature.

    Article Title: Estrogen administration attenuates post-stroke depression by enhancing CREB/BDNF/TrkB signaling in the rat hippocampus
    Article Snippet: Protein (50 μg per lane) was loaded and separated via 12% SDS-PAGE and transferred onto PVDF membranes (EMD Millipore). .. After blocking for 90 min at room temperature with 5% non-fat dried milk in TBS, the membranes were incubated overnight at 4 ° C with anti-β-actin (1:1,000; cat. no. ab8226; Abcam), polyclonal anti-CREB (1:5,000; cat. no. 9197; Cell Signaling Technology, Inc.), anti-phospho-CREB (1:5,000; cat. no. 9198; Cell Signaling Technology, Inc.) at Ser133, anti-BDNF (1:1,000; cat. no. ab6201; Abcam), anti-pTrkB (1:3,000; cat. no. 4168; Cell Signaling Technology, Inc.) or TrkB (1:3,000; cat. no. 4603; Cell Signaling Technology, Inc.) and shaken on a rotator at 4 ̊C for overnight. .. The membranes were washed three times with TBS containing 0.1% Tween-20 (TBST) for 7 min each, and subsequently incubated in HRP-labeled goat anti-rabbit secondary antibody (1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) with TBST for 1 h at room temperature.

    Incubation:

    Article Title: Estrogen administration attenuates post-stroke depression by enhancing CREB/BDNF/TrkB signaling in the rat hippocampus.
    Article Snippet: Protein (50 μg per lane) was loaded and separated via 12% SDS‐PAGE and transferred onto PVDF membranes (EMD Millipore). .. After blocking for 90 min at room temperature with 5% non‐fat dried milk in TBS, the membranes were incubated overnight at 4°C with anti‐β‐actin (1:1,000; cat. no. ab8226; Abcam), polyclonal anti‐CREB (1:5,000; cat. no. 9197; Cell Signaling Technology, Inc.), anti‐phospho‐CREB (1:5,000; cat. no. 9198; Cell Signaling Technology, Inc.) at Ser133, anti‐BDNF (1:1,000; cat. no. ab6201; Abcam), anti‐pTrkB (1:3,000; cat. no. 4168; Cell Signaling Technology, Inc.) or TrkB (1:3,000; cat. no. 4603; Cell Signaling Technology, Inc.) and shaken on a rotator at 4 ̊C for overnight. .. The membranes were washed three times with TBS containing 0.1% Tween‐20 (TBST) for 7 min each, and subsequently incubated in HRP‐labeled goat anti‐rabbit secondary antibody (1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) with TBST for 1 h at room temperature.

    Article Title: Estrogen administration attenuates post-stroke depression by enhancing CREB/BDNF/TrkB signaling in the rat hippocampus
    Article Snippet: Protein (50 μg per lane) was loaded and separated via 12% SDS-PAGE and transferred onto PVDF membranes (EMD Millipore). .. After blocking for 90 min at room temperature with 5% non-fat dried milk in TBS, the membranes were incubated overnight at 4 ° C with anti-β-actin (1:1,000; cat. no. ab8226; Abcam), polyclonal anti-CREB (1:5,000; cat. no. 9197; Cell Signaling Technology, Inc.), anti-phospho-CREB (1:5,000; cat. no. 9198; Cell Signaling Technology, Inc.) at Ser133, anti-BDNF (1:1,000; cat. no. ab6201; Abcam), anti-pTrkB (1:3,000; cat. no. 4168; Cell Signaling Technology, Inc.) or TrkB (1:3,000; cat. no. 4603; Cell Signaling Technology, Inc.) and shaken on a rotator at 4 ̊C for overnight. .. The membranes were washed three times with TBS containing 0.1% Tween-20 (TBST) for 7 min each, and subsequently incubated in HRP-labeled goat anti-rabbit secondary antibody (1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) with TBST for 1 h at room temperature.

    Control:

    Article Title: Hippocampal Oligodendrocytes Regulate Mossy Fiber Development Involved in Epileptic Responses.
    Article Snippet: .. The sources and dilutions of primary antibodies were as follows: anti-β-actin (ABclonal, AC026, 1:10000), anti-Phospho-GluR1 (S845) (HUABIO, ET1701-28, 1:700), anti- GluR1-N terminus, clone RH95 (Millipore, MAB2263, 1:3000), anti-PSD95 (Oasis, OB-PGP053, 1:1000), anti-vGLUT1 (Bioss, bs11167R, 1:1000), anti-TRKB (Abconal, A19832, 1:1000), anti-pTRKB (CST, 4168, 1:1000). β-actin served as the internal control. ..

    Article Title: Hippocampal Oligodendrocytes Regulate Mossy Fiber Development Involved in Epileptic Responses
    Article Snippet: .. The sources and dilutions of primary antibodies were as follows: anti-β-actin (ABclonal, AC026, 1:10000), anti-Phospho-GluR1 (S845) (HUABIO, ET1701-28, 1:700), anti- GluR1-N terminus, clone RH95 (Millipore, MAB2263, 1:3000), anti-PSD95 (Oasis, OB-PGP053, 1:1000), anti-vGLUT1 (Bioss, bs-11167R, 1:1000), anti-TRKB (Abconal, A19832, 1:1000), anti-pTRKB (CST, 4168, 1:1000). β-actin served as the internal control. ..

    Staining:

    Article Title: TrkB kinase activity maintains synaptic function and structural integrity at adult neuromuscular junctions
    Article Snippet: .. Antibodies anti-TrkB (Neuromics no. GT15080) and anti-pTrkB (Cell Signaling no. 4621S) stain band(s) of the appropriate molecular weight (verified in Western blot analysis of brain extracts). ..

    Article Title: TrkB kinase activity maintains synaptic function and structural integrity at adult neuromuscular junctions.
    Article Snippet: .. Antibodies anti-TrkB (Neuromics # GT15080) and anti-pTrkB (Cell Signaling 185 #4621S) stain band(s) of the appropriate molecular weight (verified in Western blot analysis of 186 brain extracts). ..

    Molecular Weight:

    Article Title: TrkB kinase activity maintains synaptic function and structural integrity at adult neuromuscular junctions
    Article Snippet: .. Antibodies anti-TrkB (Neuromics no. GT15080) and anti-pTrkB (Cell Signaling no. 4621S) stain band(s) of the appropriate molecular weight (verified in Western blot analysis of brain extracts). ..

    Article Title: TrkB kinase activity maintains synaptic function and structural integrity at adult neuromuscular junctions.
    Article Snippet: .. Antibodies anti-TrkB (Neuromics # GT15080) and anti-pTrkB (Cell Signaling 185 #4621S) stain band(s) of the appropriate molecular weight (verified in Western blot analysis of 186 brain extracts). ..

    Western Blot:

    Article Title: TrkB kinase activity maintains synaptic function and structural integrity at adult neuromuscular junctions
    Article Snippet: .. Antibodies anti-TrkB (Neuromics no. GT15080) and anti-pTrkB (Cell Signaling no. 4621S) stain band(s) of the appropriate molecular weight (verified in Western blot analysis of brain extracts). ..

    Article Title: TrkB kinase activity maintains synaptic function and structural integrity at adult neuromuscular junctions.
    Article Snippet: .. Antibodies anti-TrkB (Neuromics # GT15080) and anti-pTrkB (Cell Signaling 185 #4621S) stain band(s) of the appropriate molecular weight (verified in Western blot analysis of 186 brain extracts). ..



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    Image Search Results


    Primary and secondary antibodies for Western blotting.

    Journal: Brain Sciences

    Article Title: Ouabain Counteracts Retinal Ganglion Cell Death Through Modulation of BDNF and IL-1 Signaling Pathways

    doi: 10.3390/brainsci15020123

    Figure Lengend Snippet: Primary and secondary antibodies for Western blotting.

    Article Snippet: Rabbit anti-pTrkB , Santa Cruz Biotechnology (Dallas, TX, USA) , 1:700.

    Techniques: Western Blot